Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
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چکیده
منابع مشابه
Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
Whole-genome knockout collections are invaluable for connecting gene sequence to function, yet traditionally, their construction has required an extraordinary technical effort. Here we report a method for the construction and purification of a curated whole-genome collection of single-gene transposon disruption mutants termed Knockout Sudoku. Using simple combinatorial pooling, a highly oversam...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2016
ISSN: 2041-1723
DOI: 10.1038/ncomms13270